CDS Cloning, Sequencing and Bioinformatic Analysis of Vasoactive Intestinal Peptide in Wahui Pigeon

Abstract

Vasoactive intestinal peptide plays double roles as gastrointestinal hormone and neuropeptide. Due to few reports on vasoactive intestinal peptide in pigeon, the present study was aim to further explore the correlation between its molecular bioinformatics characteristics and biological function. After the desired RNA was extracted from the intestine of Wahui pigeons, the complete CDS of vasoactive intestinal peptide was obtained by PCR amplification and sequencing, in addition, a variety of bioinformatics analysis was performed to evaluate its structural characteristics. CDS of vasoactive intestinal peptide, encoding 132 amino acids, is 399 bp long in Wahui pigeon. Vasoactive intestinal peptide probably belongs to a hydrophilic peptide without any signal peptide and transmembrane structure, and mainly plays its biological function due to occupying 89.6% in cytoplasm. Furthermore, it consists of one N-glycosylation site and 17 phosphorylation sites, and the secondary structure contains 50.76% of alpha helix, 5.30% of beta turn, 9.09% of extended strand, and 34.85% of random coil. Phylogenetic tree analysis shows that Wahui pigeon hold the close relative characteristics with chicken, turkey, and Taeniopygia guttata. Besides its conservative evolutional characteristic, the simple structure, subcellular localization, hydrophobicity and hydrophilicity indirectly account for its extensive distribution and various functions as expected.