Integrin αVβ5 is not involved in adeno-associated virus type 2 (AAV2) infection

Abstract

αVβ5 integrin was recently proposed as a coreceptor for adeno- associated virus type 2 (AAV2) infection (Summerford et al., 1999, Nat. Med. 5, 78-82), based mainly on the direct binding of AAV2 to denatured β5 by virus overlay assay. In studies using purified natural or recombinant human integrin αVβ5 we were unable to demonstrate AAV2 binding, either by virus overlay or by liquid binding assay. Furthermore, neither purified integrin αVβ5, nor RGD peptides, nor functional blocking monoclonal antibody blocked rAAV2 transduction. These data strongly suggest that integrin αVβ5 is not involved in AAV2 infection.