Leptin promotes the growth of breast cancer by upregulating the wnt/β-catenin pathway

Abstract

The present study aimed to investigate the role of leptin in the growth of breast cancer. In the present study, expression of leptin in tumor tissues and adjacent tissues was detected by reverse transcription-quantitative polymerase chain reaction. Serum leptin in patients with breast cancer and normal healthy controls was detected by ELISA. Different concentrations of leptin were used to treat breast cancer cells, and cell proliferation was detected by cell proliferation assay. Interactions between leptin and Wnt/β-catenin were evaluated by western blotting. It was demonstrated that expression of leptin mRNA was significantly higher in tumor tissues than in adjacent tissues in 43 out of 48 patients. Serum level of leptin was significantly higher in patients with breast cancer than in normal controls. Leptin promoted the proliferation of breast cancer cells in a dose-dependent manner. Leptin significantly increased the expression level of β-catenin in breast cancer cells. Treatment with Wnt inhibitor reduced the enhancing effects of leptin on proliferation of breast cancer cells. The results of the present study therefore indicate that leptin may promote the growth of breast cancer by upregulating the Wnt/β-catenin pathway.