Adaptation of the acid citrate agar gel method for hemoglobin electrophoresis

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Abstract

We describe adaptation of the acid citrate agar method for separation of hemoglobin variants. Eight hemolysates, applied to agar supported in a plastic tray, are electrophoresed for 50 min at room temperature. Materials are inexpensive, and normal and commonly encountered abnormal hemoglobins are separated into sharp, concise zones.

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LeCrone, C. N., Broszeit, H. K., Jones, J. A., & Detter, J. C. (1976). Adaptation of the acid citrate agar gel method for hemoglobin electrophoresis. Clinical Chemistry, 22(10), 1743–1744. https://doi.org/10.1093/clinchem/22.10.1743

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