Disubstituted 4-chloro-3-nitrophenylthiourea derivatives: antimicrobial and cytotoxic studies

Abstract

4-Chloro-3-nitrophenylthioureas 1-30 were synthesized and tested for their antimicrobial and cytotoxic activities. Compounds exhibited high to moderate antistaphylococcal activity against both standard and clinical strains (MIC values 2-64 g/mL). Among them derivatives with electron-donating alkyl substituents at the phenyl ring were the most promising. Moreover, compounds 1-6 and 8-19 were cytotoxic againstMT-4 cells and various other cell lines derived from human hematological tumors (CC50 10 M). The influence of derivatives 11, 13 and 25 on viability, mortality and the growth rate of immortalized human keratinocytes (HaCaT) was observed.