Genetic characterization of hazelnut (Corylus avellana L.) cultivars from Turkey using molecular markers

Abstract

Genetic relationships among 18 Turkish hazelnut (Corylus avellana L.) cultivars were investigated using randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) markers. Twenty-five RAPD primers, 25 ISSR primers, and eight AFLP primer pairs generated a total of 434 polymorphic marker loci. The three marker systems were able to differentiate the cultivars. Genetic similarity index values ranged from a high of 0.96 for 'Kan' and 'UzunMusa' to a low of 0.73 for 'Yassi Badem' and 'Kalinkara'. The genetic relationships were presented as an unweighted pair group method with arithmetic average (UPGMA) dendrogram and a three-dimensional principal coordinate analysis (PCoA) plot. The UPGMA dendrogram showed two main clusters, while PCoA analysis showed three groups. Cultivar-specific markers were produced by all marker systems for 10 cultivars. This study demonstrates the usefulness of molecular markers for identification of hazelnut cultivars.