Micropropagation of calla lily (Zantedeschia rehmannii)

Abstract

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm-3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm-3). Cultures were maintained in darkness. Soaking explants in an L-ascorbic acid solution improved the establishment of explants. Culture initiation should be conducted on media supplemented with 3 mg dm-3 BAP. On a multiplication stage, adventitious shoots were placed on MS media supplemented with cytokinin: BAP (0.5 to 5 mg dm-3), KIN (0.5 to 5 mg dm-3), TDZ (0.1 to 1 mg dm-3) and 2iP (2.5 to 15 mg dm-3) or BAP (0.5 to 7.5 mg dm-3) with IAA (0.5 to 2 mg dm-3). The highest coefficient of multiplication for Zantedeschia was obtained on the medium with the addition of 2.5 mg dm-3 BAP, which positively affected the shoot length (3.41 cm) and the number of adventitious shoots (4.13). Rooting took place on media supplemented with IBA, IAA and NAA at a concentration of 0.1 to 2 mg dm-3. The most numerous and the longest roots were found in plants placed on a medium with the addition of 0.1 mg dm-3 IBA.