Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants

Abstract

Crude juices of Sidr (Zizyphus Spina-Christi L.) from leaves were obtained by hydraulic press. The levels of polyphenolic compounds in the (leaves) juice were 510.00 and 722.00ppm. Aliquots of the concentrated sidr juice (leaves), represent 200, 400, 800 and 1600ppm and butylated hydroxyl toluene (BHT, 200ppm) were investigated by Rancimat method at 100ºC and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. These compounds were administrated to rats daily for 6 weeks by stomach tube. The liver (Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities) and kidney (bilirubin, uric acid and creatinine) function tests and serum contents (total lipids, total cholesterol and low and high-density lipoproteins) were measured to assess the safety limits of the phenolic compounds in the sidr juice (fruits and leaves). The data of the aforementioned measurements indicates that the administration of sidr juice (leaves) did not cause any changes in liver and kidney functions. On the contrary, BHT at 200ppm induced significant increases in the enzyme activities and the serum levels of total lipids, uric acid and creatinine.