Synthesis and evaluation of PEG derivatives for crosslinking cells

Abstract

The objective of this study is synthesis, characterization, and evaluation of a novel crosslinker to form spheroids. Our approach was based on the crosslinking of cell membrane using a polymeric crosslinker via hydrophobic interaction. A crosslinker polyethylene glycol) derivative with oleyl groups as hydrophobic group at both ends was synthesized, and then characterized with gel permeation chromatography and Fourier-transform infrared spectroscopy. Furthermore, cell culture experiments were performed to confirm spheroid formation. Spheroids were successfully obtained when the crosslinker was added to cell suspension. This polymeric crosslinker was useful in the formation of cell spheroid. Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA.