Molecular genotyping of enterococcus faecalis isolated from clinical sources by BOX-PCR and ERIC-PCR

Abstract

Gram-positive bacteria, Enterococcus faecalis, cause serious nosocomial infections such as UTI, bloodstream infections, and endocarditis. The study's aimed  to isolate and identify Enterococcus faecalis from various clinical specimens in Al-Diwanyiah province hospitals ,testing the sensitivity of isolates to antibiotics  as well as to genotyping of E.faecalis  isolates by BOX –PCR or ERIC-PCR Analysis. The study extended from October 2021 to January 2022. Biochemical tests, Vitek 2 and 16S rRNA analysis identified 37 bacteria as Enterococcus faecalis. Five clusters (I-V) and about 13 polymorphic variants showed among 20 isolates by ERIC PCR. Whereas, four cluster (I-IV) and 14 polymorphic variants were showed by BOX PCR. The results showed that that there is a slight difference between ERIC and BOX-PCR analysis for Enterococcus faecalis isolated from clinical samples.