Stigmasterol content of Artemisia annua L. And the phytosterol profile

Abstract

Objective: To find the stigmasterol source of Artemisia annua L. and to study the profile of phytosterol types in A. annua hairy root cultures. Methods: Stigmasterol content determination was done based on stigmasterol content in the methylene chloride extract of A. annua using high performance liquid chromatography in wild-type plant, plant tissue cultures, and hairy root cultures. The optimal resolution was achieved by suitability system, and stigmasterol calibration curve was generated with stigmasterol concentrations series. Phytosterol types in A. annua hairy root cultures were performed by gas chromatography-mass spectrophotometry. Results: Highest stigmasterol content in A. annua is shown by hairy root cultures of A. annua in the 5th week harvesting time, which is 1.5 g/100 g (w/w) extract. The phytosterol profile in A. annua hairy root cultures encompassed stigmasterol 74.6%, β-sitosterol 13.5%, and campesterol 11.9%. Conclusion: A. annua hairy root cultures in 5-week harvesting time prove its potential as stigmasterol source alternative. The highest phytosterol type in A. annua hairy root cultures is stigmasterol. This is the first report stigmasterol content and its phytosterol profile of A. annua hairy root cultures extract.