Antibacterial activity and characterization of Annona muricata Linn leaf extract-nanoparticles against Escherichia coli FNCC-0091 and Salmonella typhimurium FNCC-0050

Abstract

The objective of this study was to evaluate the characterization and inhibitory effects of Annona muricata Linn leaf extract loaded by chitosan nanoparticles on the growth of Escherichia coli FNCC-0091 and Salmonella typhimurium FNCC-0050. The chitosan-sodium tripolyphosphate-nano particles (CS-TPP-NPs) were prepared by ionic cross-linking of CS-TPP and were characterized physico-chemically (particle size, zeta potential) and morphologically. Antibacterial activity of the CS-TPP-NPs-AmE was performed by well diffusion method. The optimum formula for CS-TPP-NPs was analyzed using centrifugation at 3,500 rpm for 30 min. A wide range, 1:1:0.02 to 1:1:0.01 of CS:AmE:TPP ratios were evaluated with constant CS. Data obtained for bacterial activities were statistically analysed using one-way analysis of variance (ANOVA). The means between groups were separated with Duncan post-hoc test. The result showed that the optimum formula for CS-TPP-NPs of AmE was 1:1:0.01. The optimum formula of CS-TPP-NPs-AmE was characterized by particle size, zeta potential, and morphology. Results showed that the particle size of CS-TPP-NPs-AmE was 234.00 ± 21.5 nm with spherical shape and positive charges on their surface with the zeta potentials 34.77 ± 4.97 mV. CS-TPP-NPs of AmE in the ratio 1:1:0.01 has efficient antibacterial activities against E. coli FNCC-0091 and S.Typhimurium FNCC-0050.