Potensi Bakteri Agens Hayati untuk Menekan infeksi Cucumber mosaic virus (CMV) pada Melon (Cucumis melo L.)

Abstract

Cucumber mosaic virus (CMV) is an important pathogen of Cucurbitaceae, including melons. Virus management in the field is difficult to do. One of effort way to reduce viral infections is through improve plant health by using bacterial biological agents. This research was done to test the potential of bacterial isolates of Bacillus cereus (BC), Chromobacterium haemolyticum (CH), Ralstonia pickettii (RP), and Pseudomonas flourescen (PF) in suppressing CMV infections on melon plants. Bacteria effect testing through the performance of seedling was done by soaking the seeds in the bacterial suspension dan growing on test in laboratory. While efficacy of bacteria in suppressing CMV infection was conducted by soaking the seeds and spraying the plant with bacterial suspension in a greenhouse trial. Observations were measured on the disease and agronomic parameter, and peroxidase enzyme activity. The result showed that the treatment of BC bacteria showed the best germination performance among other bacteria treatment. Bacterial treatment did not affect the incubation period and the incidence of the disease, but it significantly decreases the severity of the disease and viral accumulation in compared with control. Peroxidase enzyme activity was higher after bacterial treatment and CMV inoculation in compared to treatment before inoculation. RP bacteria are the best bacteria in suppressing CMV infection through antiviral activity and induction of plant defense by increased peroxidase activity.