Bovine serum as an alternative to control serum for total protein levels

Abstract

BACKGROUND: Total protein describes the liver’s ability to synthesize protein and metabolize substances in the blood. AIM: We aimed to investigate whether cow serum can be used as a control agent because the analyte in bovine blood serum is almost the same as that of humans. METHODS: Homogeneity and stability of bovine serum by adding 7.5% ethylene glycol preservative after being stored for 12 weeks at −20°C. This study uses a one-group pretest-posttest design. The alternative serum is derived from cow blood waste at a slaughterhouse in Giwangan, Yogyakarta, Indonesia. Data analysis was based on ISO 13528:2005 regarding stability and homogeneity of control serum. RESULTS: From the examination of the total protein level in the serum, the value of Xr–Yr = 0.23817; this value meets the test criteria |Xr–Mr| 0.3σ, that is 0.23817 ≤ 0.91585. CONCLUSION: Based on the results, serum from bovine can be an alternative to assess total protein levels and is still stable and homogeneous even though it is stored for 12 weeks.