Dissection of Laboratory Animal and Sample Collection for Histology

Abstract

The starting point for the laboratory investigation of a dissection of laboratory animal for experiment is the taking of samples. This review considers some general principles involved in sample collection for histology (Liu et al., 2016). For disease diagnosis, the tissues sampled should be representative of the condition being investigated and the lesions observed. Samples should be taken with care, to avoid undue stress or injury to the animal or danger to the operator. Where appropriate, samples should be collected aseptically, and care should be taken to avoid crosscontamination between samples (Lapage, 1958). Mice and rats are the most used animals in experimental researches, the anatomical, histological and genetic differences between species should be carefully evaluated, to better apply the study model and avoid unnecessary waste avoid (Corte et al., 2021). Currently, the implantation of defined genetically and sanitarily laboratory animals has aided in new discoveries, through experimental models, contributing to the prevention of uncured diseases such as cancers, AIDS, and multiple sclerosis, and also for the development of new surgical treatment techniques. Other applications correspond to the vaccines development, monoclonal antibodies, evaluation and control of biological products, pharmacology, toxicology, bacteriology, virology and parasitology, and basic immunology studies, immunopathology, organ transplants and immunosuppressive drugs development. However, with technological advances, it is now possible to obtain satisfactory results through alternative methods in vitro, using cell culture and other methods, allowing the 84 replacement of laboratory animals (Gunatilake, 2018).