Cysteine promoted C-terminal hydrazinolysis of native peptides and proteins

Abstract

Tagging the terminus: N→S acyl transfer in native peptides and proteins can be reliably intercepted with hydrazine. The method allows selective labeling and ligation, without recourse to the use of protein-splicing elements. NCL=native chemical ligation. © 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.