MiR‑589‑5p is downregulated in prostate cancer and regulates tumor cell viability and metastasis by targeting CCL‑5

Abstract

Prostate cancer is one of the most common human malignancies, which represents a serious threat to health, and microRNAs (miRNAs/miRs) have been reported to be closely associated with the progression and development of prostate cancer. The present study aimed to investigate the expression patterns, functions and underlying mechanisms of miR-589-5p in prostate cancer. The results demonstrated that the expression levels of miR-589-5p were downregulated in prostate cancer tissues and cell lines. Overexpression of miR-589-5p inhibited cell viability, migration and invasion in prostate cancer cells. Subsequently, chemokine (C-C motif) ligand 5 (CCL‑5) was identified as a direct target gene of miR‑589‑5p, which was highly expressed at the mRNA and protein levels in prostate cancer tissues and cells. Furthermore, CCL-5 mRNA was negatively correlated with miR-589-5p expression in prostate cancer tissues. Silencing CCL-5 promoted the apoptosis, and inhibited the migration and invasion of prostate cancer cells. Taken together, these results indicated that miR-589-5p may act as a tumor suppressor in prostate cancer by targeting CCL-5, thus suggesting that miR-589-5p may be a novel and reliable molecular marker for the diagnosis and prognosis of prostate cancer.