Intra- and extracellular element concentrations of rat renal papilla in antidiuresis

Abstract

The element concentrations in various intra- and extracellular compartments of the tip of the rat renal papilla were determined during antidiuresis using electron microprobe analysis. Urinary concentrations (means ± SEM) were: urea, 1509 ± 116; potassium, 268 ± 32; sodium, 62 ± 19 mmoles·1-1; and osmolality, 2548 ± 141 mOsm·kg-1. Electrolyte concentrations in the interstitial space were: sodium, 437 ± 19; chloride, 438 ± 20; and potassium, 35 ± 2 mmoles·kg-1 wet wt. The vasa recta plasma exhibited almost identical element concentrations. The values in the papillary collecting duct cells were: sodium, 28 ± 1; chloride, 76 ± 3; potassium, 135 ± 3; and phosphorus, 316 ± 7 mmoles·kg-1 wet wt. Similar concentrations were observed in the papillary epithelial cells. In interstitial cells potassium and phosphorus concentrations were virtually identical to those of the collecting duct cells, whereas sodium and chloride concentrations were higher by about 30 mmoles·kg-1 wet wt. The element composition of the various papillary cells is, thus, not substantially different from that of proximal tubular cells. This finding demonstrates that cellular accumulation of electrolytes is not the regulatory mechanism by which papillary cells adapt osmotically to their high environmental osmolality and sodium chloride concentration.