Chlorogenic acid from banana and papaya peels inhibit lipid accumulation in 3T3-L1 cells

Abstract

The aim of this study was to determine the capability of chlorogenic acid (CGA) from banana (Saba) and papaya (Caricaceae) peels to inhibit lipid accumulation. Total phenolic content (TPC), CGA content, cytotoxic activity, and lipid profiling for both ethanolic and water extracts of both fruit peels at ripe (Sbr and Cpr) and unripe (Sbur and Cpur) stages were conducted. TPC for Sbr water extract (20.66 mg GAE/g DW) was significantly higher than that of the Sbr ethanolic extract (2.88 mg GAE/g DW). CGA was significantly higher in Cpr water extract (10.08 mg/L) compared to ethanolic extract (5.54 mg/L). Cytotoxic activity of the extract was determined by MTT assay using 3T3-L1 pre-adipocyte cell lines. Lipid study was assessed by Oil Red-O staining and adipolysis assay. All of the extracts showed no cytotoxic effect towards the adipocyte cells. The extracts significantly reduced lipid accumulation and had higher adipolysis rate compare to control, indicating the reduction in lipid production in 3T3-L1 adipocyte cell lines.(Abbreviations: Sbr: Saba banana ripe; Sbur: Saba banana unripe; Cpr: Caricaceae papaya ripe; Cpur: Caricaceae papaya unripe.)