Effect of medium composition on the production of anthocyanins by hairy root cultures of Ipomoea batatas

Abstract

We obtained hairy roots of Ipomoea batatas cv. Ayamurasaki by infection of Agrobacterium rhizogenes A13. Accumulation of anthocyanins in hairy roots was observed when they were cultured under continuous light irradiation, whereas it was not observed when they were cultured in the dark. The amount of accumulated anthocyanins in hairy roots increased as a result of choosing the medium and increasing the sucrose concentration in the medium. Accumulation of anthocyanins was also observed in the hairy roots cultured in liquid medium in the dark by choosing medium.