Hydrolysis of Glycerol Trioleate and Extraction of Its Fatty acid Under C02 Supercritical Conditions

Abstract

Hydrolysis of glycerol trioleate used as a standard sample of lipid and the extraction of its fatty acid were carried out under supercritical conditions of CO2 in order to develop a conventional technique to recover fatty acids in algae. Glycerol trioleate was hydrolyzed successfully under the tempreture of 250 °C and pressure of 8 MPa by adding water to the supercritical fluid of CO2. In the conditions glycerol and oleic acid was not extracted in the CO2 fluid. After the hydrolysis the oleic acid was extracted in high efficiency at the CO2 supercritical fluid density higher than 0.55 g/cm3 without accompanying glycerol extraction in which the temperature of 80 °C and the pressure of 20 MPa was selected as one of the extraction conditions. No degradation of the fatty acid exposed to the hydrolysis and extraction conditions were observed by liquid chromatographic analysis. From above results, it was found that the process of hydrolysis of lipid followed the extraction of their fatty acids was easily performed by changing only temperature and pressure in one vessel. © 1995, The Chemical Society of Japan. All rights reserved.