ASSESSMENT OF PHYTOCHEMICAL AND PHARMACOLOGICAL PROPERTIES OF ETHANOLIC EXTRACT OF CERBERA MANGHAS L. LEAVES

Abstract

The objective of the present study was to investigate the phytochem. nature (group detn. of plant constituents) as well as the antioxidant and analgesic activity of ethanolic ext. of the Cerbera manghas L. leaves (Family: Apocynaceae). Phytochem. anal. of ethanolic ext. of Cerbera manghas leaves indicated the presence of carbohydrate (reducing sugars), alkaloids, tannins, steroids, flavonoids & glycosides. The ext. exhibited significant (P<0.01) analgesic effect in acetic acid induced writhing of white albino mice at the dose of 500 mg/kg body wt. The ext. inhibited 30.65% writhing in mice and it is comparable to the activity of the std. analgesic drug Diclofenac sodium (79.03% P<0.001) at the dose of 25 mg/kg body wt. The ext. showed the antioxidant property by the presence of strong yellow spot on a purple background on the TLC plate and in the free radical scavenging activity study (DPPH assay) the ext. showed significant antioxidant activity (IC50 ∼ 269.15 μg/mL) which is comparable to std. (Ascorbic Acid, IC50 ∼ 14.45 μg/mL). The findings suggest that the ethanolic ext. of Cerbera manghas leaves possess antioxidant and analgesic activities. [on SciFinder(R)]