A procedure for the analysis of s-benzyl-n-acetylcysteine and s-(o-methylbenzyl)-n-acetylcysteine in human urine

Abstract

1. Analytical methods for mercapturic acids, the end-products of gluthathione metabolism of the industrial solvents toluene and o-xylene (i.e., S-benzyl-N-acetylcysteine and S-(o-methylbenzyl)-N-acetylcysteine), added to human urine have been developed. 2. The sensitivity is 2ng/μl (R.S.D. = ±13% and 1 ng/μl (R.S.D.= ±6.4% urine for S-benzyl-N-acetylcysteine and S-(o-methylbenzyl)-N-acetylcysteine, respectively, when using the h.p.l.c. method. 3. The corresponding sensitivities for the g.l.c.-mass spectrometric method are 0.5 and 0.3 ng/μl urine, respectively. © 1986 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.