Antibiotic resistance and sero-groups of Shigella among paediatric out-patients in Southwest Ethiopia

Abstract

To determine the prevalence of Shigella sero-groups and resistance pattern of isolates to commonly used antibiotics in Jimma. Cross-sectional survey. The study was conducted in Jimma, southwest Ethiopia. A total of 384 paediatric out-patients with diarrhoea aged 14 years and below were studied. Stool specimens were collected from children presenting with diarrhoea using Cary-Blair transport medium and buffer treated swabs from Jimma hospital and Jimma health centre. Isolation, biochemical characterisation, sero-grouping and antibiotic sensitivity testing were performed according to standard methodology in the Microbiology laboratory of Jimma University. Out of the 77 Shigella strains isolated, sero-group A comprised 29.9%, B 40.3%, C 19.5 % and D 10.4 %. Among all Shigella sero-groups, highest resistance was encountered to tetracycline (63.6%), ampicillin (70.1%), cephalothin (57.1%), trimethoprim-sulphamethoxazole (32.5 % ) and chloramphenicol (40.3 %) while least resistance was observed to gentamicin (1.3%), polymyxin B (3.9%) and nalidixic acid (6.5%). Gentamicin, polymyxin B and nalidixic acid were found to be the drugs of choice for cases related with shigellosis.