Tannin nanoparticles (Np99) enhances the anticancer effect of tamoxifen on er+ breast cancer cells

Abstract

Recently, natural substances in the form of nanoparticles are increasingly being used in different field, particularly in medicines to enhance their beneficial effects in treatment and prevention. Cancer cells of the breast (MCF-7) have been chosen to be examined and treated in vitro with conventional drug Tamoxifen (Tam) and tannin nano-particles extract (NP99) individually or in combination. MTT reagent has been applied to assess the cell viability and propagation percentage, DNA fragmentation and mRNA relative expression of apoptotic genes to study the cell death pathway. The results showed that Tam and tannin NP99 triggered cytotoxic activity towards the MCF-7 cell. They reduce the viability and induced high potent repressive activity on cell proliferation percentage and induced apoptosis as indicated by rising the percentage in DNA fragmentation. Effect of NP99 extract exhibited its effect in a dose and time-varying. The combined treatment of Tam and NP99 were much more efficient than individual drugs. It could be concluded that NP99 is considered a promising natural anticancer agent as a new tool in therapeutic strategies.