Quantitative changes in the mRNA for contractile proteins and metabolic enzymes in masseter muscle of bite-opened rats

Abstract

To study the effects of bite opening on the fibre phenotypes of rat masseter, the mRNAs of four predominant myosin heavy-chain isoforms (MHC I, IIa, IId/x and IIb) and two alkali light-chain isoforms (LC1f and 3f) as well as those of two metabolic enzymes, carbonic anhydrase III (CAIII, oxidative enzyme) and glucose-phosphate isomerase (GPI, glycolytic enzyme), were measured in relation to the total RNA of masseter muscle by competitive, reverse transcriptase-polymerase chain reaction in control and bite-opened rats. Bite opening (2.8 mm increase in the vertical dimension for 1 week) significantly (P < 0.05) increased the amount of MHC IIa mRNA but decreased (P < 0.001) the amount of MHC IIb mRNA without changing the amount of MHC IId/x mRNA. No MHC I mRNA was found in any masseter studied. A significant (P < 0.01) increase in the mRNA of LC1f associated with a decrease (P < 0.05) in that of LC3f was observed after the bite opening. The CAIII mRNA increased significantly (P < 0.001), while the GPI mRNA decreased (P < 0.05) in association with the bite opening. These results strongly suggest that in I week of bite opening changes the rat masseter muscle from a glycolytic, MHC IIb-LC3f-dominant fibre to an oxidative, MHC IIa-LC1f-dominant fibre. (C) 2000 Elsevier Science Ltd.