Isolation and Characterization of Pepsin-Solubilized Collagen from the Skin of Black Carp ( Mylopharyngdon piceus)

Abstract

To make more effective use of underutilized resources, pepsin-solubilized\rcollagen (PSC) was successfully extracted from the skin of black carp (Mylopharyngdon piceus) with a yield of 45.7%\rbased on dry weight. The PSC comprising two identical α1-chains and one α2-chain\rwith no disulfide bond was characterized as type I, and it contained 195 imino acid\rresidues vs. 1000 amino acid residues. The collagen showed an absorption edge around\r218 nm, which was lower than the maximum absorption wavelength of other PSC. The\rdenaturation temperature of PSC was 25.6°C, which was lower than that of porcine\rcollagen by approximately 11°C. The isoelectric point (pI) was estimated to be 8.23,\rand the collagen was soluble at an acidic pH as well as below 40 g/L NaCl. It is\rthought that the high yield and stability of PSC from the skin of black carp warrant\rits application as a new source of collagen for industrial purposes.