Sequential staining of euchromatic and heterochromatic regions of the human Y chromosome

Abstract

A sequential silver-Giemsa (SG) procedure is presented, initially to stain the p11 and q11 euchromatic bands and subsequently the q12 heterochromatic band of the human Y chromosome. A three sub-band division of the q11 band can be identified. The same technique differentially stains the secondary constriction of chromosome 9 as well as most of the other satellite III DNA regions of the human karyotype.