The comparative analysis of infection pattern and oocyst output in Eimeria tenella, E. maxima and E. acervulina in young broiler chicken

Abstract

Aim: To assess the pattern of infection pattern by artificially infected Eimeria strains of Korean isolates and assessed the degree of sporulating oocysts at different temperature. Materials and Methods: Birds were orally inoculated with oocysts of Eimeria tenella, Eimeria maxima and Eimeri acervulina. Oocyst count, oocyst isolation and sporulation were evaluated from the fourth to the tenth day post infection. Histopathological studies also made in the caecum and intestinal to comparative lesions. Results: Mean oocyst counts of these species increased more quickly on the day of 5th to 6th of post-infection. E. acervulina reached its highest infection level on the 6th day while both E. tenella and E. maxima had peak on the 7th day. The prepatent period of oocyst output in the E. acervulina storied 120 hand ended on the th day after inoculation, whereas the E. maxima started oocyst output at 144 h and E. tenella oocyst output at 168 h respectively. The best temperature for optimum sporulation was found to be at25°C at sporulation rate of 88.91%, while at20°C sporulated 88.03%, and at 30°C sporulated 82.44%. Conclusion: Current study represent the pattern of infection, pathogenesis and optimum sporulation temperature and our results suggested that 25°C is optimum for sporulated oocyst of Korean isolates oiE. tenella, E. maxima andis. acervulina. © The authors.