Induction of Extracellular Lytic Enzymes by Fusarium solani

Abstract

Fusarium solani is a necrotrophic parasitic fungus that causes wilt in some plants, causing severe economic losses in some areas of the country. The objective of this work was to analyze the induction of extracellular lytic enzymes pro-duced by a strain of F. solani, isolated from a culture of tomato, in Villa de Arista, S.L.P. México. Polygalacturonase activity has a greater induction time at 10 days, and the xylanase has two times higher activity at 8 and 13 days of incu-bation at 28˚C. Also, the xylanase activities A and B were very stable at 4˚C. After 7 days of incubation, it has an activ-ity of 100% and 96%, respectively, while polygalacturonase retains 61% of its initial activity. Both activities are better induced with glutamate and urea as nitrogen sources respectively, and both exhibit an initial pH optimum of 5.5. Fi-nally, we didn't find cellulase activity in the analyzing conditions.