Specific chemical labeling of DNA fragments

Abstract

We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termin1. The procedure includes enzymatic addition of 4-th1ourid1ne, followed by reaction in mild non-denaturing conditions with the highly reactive α-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with β-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution. © 1979 Information Retrieval Limited.