Current knowledge for the western blot normalization.

Abstract

Western blot (WB) is a fundamental technique in cell and molecular biology, and cancer biology research, used to separate and identify cellular proteins. The techniques and procedures for WB have progressed over the last 30 years. However, there is still debate whether to perform protein normalization using housekeeping proteins or total protein normalization. Here, the key factors for WB normalization methods are summarized and potential areas for future research are presented.