Comparative transcriptomics reveals shared gene expression changes during independent evolutionary origins of stem and hypocotyl/ root tubers in Brassica (Brassicaceae)

Abstract

Plant succulence provides a classic example of evolutionary convergence in over 40 plant families. If evolutionary parallelism is in fact responsible for separate evolutionary origins of expanded storage tissues in stems, hypocotyls, and roots, we expect similar gene expression profiles in stem and hypocotyl / root tubers. We analyzed RNA-Seq transcript abundance patterns in stem and hypocotyl / root tubers of the Brassica crops kohlrabi (B. oleracea) and turnip (B. rapa) and compared their transcript expression profiles to those in the conspecific thin-stemmed and thin-rooted crops flowering kale and pak choi, respectively. Across these four cultivars, 38,192 expressed gene loci were identified. Of the 3,709 differentially-expressed genes (DEGs) in the turnip: pak choi comparison and the 6,521 DEGs in the kohlrabi: kale comparison, turnips and kohlrabies share a statistically disproportionate overlap of 841 DEG homologs in their tubers (p value < 1e-10). This overlapping set is statistically enriched in biochemical functions that are also associated with tuber induction in potatoes and sweet potatoes: sucrose metabolism, lipoxygenases, auxin metabolism, and meristem development. These shared expression profiles in tuberous stems and root / hypocotyls in Brassica suggest parallel employment of shared molecular genetic pathways during the evolution of tubers in stems, hypocotyls and roots of Brassica crops and more widely in other tuberous plants as well.