Modified oligonucleotides as a tool for DNA sequencing, fingerprinting and mapping

Abstract

The synthesis of modified oligonucleotides bearing 5-methylcytosine and 2-aminoadenine instead of natural counterparts, which are characterised by enhanced strength of complementary DNA binding (strong binding oligonucleotides, SBO) has been developed. Advantages at SBO applications are described as hybridisation probes, probes for physical mapping, primers for DNA sequence and PCR amplification.