The impact of the humate nature feed additives on the antioxidative status of erythrocytes, liver, and muscle in chickens, hens, and gerbils

Abstract

Our study's goal was to determine the oxidation states of formed elements of the blood, and mitochondria of liver and muscles of chicken and gerbils could help establish and compare the humic substance's impact. The experiment was carried out on hens of the Lohmann Braun, chicken-broilers of the Cobb 500 cross, and 6-months-old gerbils, which were kept under standard conditions. Experimental hens, chicken-broilers, and gerbils were divided into 2 groups: 1 – intact animals (control), 2 – animals, which added to the drinking water a 1% solution of the fodder biologically active additive of humic nature (Humilid or Hydrohumate) in the optimal amount for 21 days. Results showed that Humilid and Hydrohumate tested in our experiment activated the protective systems in cells against toxic radicals. The species-specific effects of the components of studied humates were established: the activation of the glutathione blood protection system of hens; catalase activation in mitochondria of white muscles of chicken-broilers; activation of catalase and increase of concentration of cytochrome C in liver mitochondria of gerbils. Our results indicate the elucidation of the mechanisms of humic.