Isothiocyanates produced by Brassicaceae species as inhibitors of Fusarium oxysporum

Abstract

Glucosinolates contained in members of the Brassicaceae release isothiocyanates potentially useful in controlling Fusarium oxysporum pathogens in conifer seedling nursery soils. Our objective was to determine the toxicity of individual isothiocyanates to different growth stages of the fungus. Bioassays with four F. oxysporum isolates were conducted using sealed containers in which 0.3 μl of 2-propenyl, ethyl, buty, phenylethyl, benzyl, or phenyl isothiocyanate was allowed to volatilize. Propenyl and ethyl isothiocyanates were the most fungistatic of those compounds tested. The same concentrations of propenyl and ethyl isothiocyanates that inhibited mycelial growth completely suppressed conidial and chlamydospore germination of all isolates. Other isothiocyanates including ethyl, benzyl, and phenethyl were also fungitoxic to F. oxysporum conidia and chlamydospores. Reduction in pathogen populations resulting from a green-manure crop are likely achievable since chlamydospores are sensitive to isothiocyanate. Pathogenic F. oxysporum isolates infesting nursery soils would likely be most suppressed by species of plants such as Brassica carinata, B. nigra, and B. juncea, which contain glucosinolates that release high concentrations of propenyl isothiocyanate.