Effects of phosphatidylserine-containing liposomes on odontogenic differentiation of human dental pulp cells

Abstract

Phosphatidylserine (PS) is known to enhance biomineralization due to the ability to accumulate calcium ions. In this study, the effects of PS on odontogenic differentiation and mineralization of human dental pulp cells (HDPCs) were investigated using phosphatidylserine-containing liposomes (PSLs). PSL was slightly cytotoxic at 125 µM in growth medium, and ALP activity was up-regulated in the PSL-treated HDPCs at non-cytotoxic concentrations. Mineralization was also enhanced by PSL, while mRNA expressions of DSPP and OCN genes were slightly attenuated. The mRNA expression of Runx2 was not altered by PSL. It is thus likely that PSL selectively affected odontogenic differentiation processes of HDPC. Finally, the interaction between PSL and HDPC was investigated by staining with annexin V-FITC in PSL-treated HDPC. It was found that PS was gradually incorporated into HDPC cytoplasm for several days. The results of this study suggest that PSL is able to stimulate dentin formation in dental pulps.