L-Rhamnose metabolism in Clostridium beijerinckii strain DSM 6423

Abstract

Macroalgae (or seaweeds) are considered potential biomass feedstocks for the production of renewable fuels and chemicals. Their sugar composition is different from that of lignocellulosic biomasses, and in green species, including Ulva lactuca, the major sugars are L-rhamnose and D-glucose. C. beijerinckii DSM 6423 utilized these sugars in a U. lactuca hydrolysate to produce acetic acid, butyric acid, isopropanol, butanol, and ethanol (IBE), and 1,2-propanediol. D-Glucose was almost completely consumed in diluted hydrolysates, while L-rhamnose or D-xylose was only partially utilized. In this study, the metabolism of L-rhamnose by C. beijerinckii DSM 6423 was investigated to improve its utilization from natural resources. Fermentations on D-glucose, L-rhamnose, and a mixture of D-glucose and L-rhamnose were performed. On L-rhamnose, the cultures showed low growth and sugar consumption and produced 1,2-propanediol, propionic acid, and n-propanol in addition to acetic and butyric acids, whereas on D-glucose, IBE was the major product. On a D-glucose- L-rhamnose mixture, both sugars were converted simultaneously and L-rhamnose consumption was higher, leading to high levels of 1,2-propanediol (78.4 mM), in addition to 59.4 mM butanol and 31.9 mM isopropanol. Genome and transcriptomics analysis of D-glucose- and L-rhamnose-grown cells revealed the presence and transcription of genes involved in L-rhamnose utilization and in bacterial microcompartment (BMC) formation. These data provide useful insights into the metabolic pathways involved in L-rhamnose utilization and the effects on the general metabolism (glycolysis, early sporulation, and stress response) induced by growth on L-rhamnose.