Ethanolic Extract of Moringa oleifera Leaves Ameliorates TNF- and IL-8 Level from Rat Model of S. Aureus-Induced Rhinosinusitis

Abstract

Background: Acute bacterial rhinosinusitis is a common complication that require antibiotic therapy to recover promptly. To avoid the emergence and spread of antibiotic-resistant bacteria, Ethanol extract of moringa leaves (EEDK) contains active ingredients that serve as antioxidants that can help restore redox metabolic balance and can reduce excess free radicals so as to prevent oxidative stress and speed healing. Materials and Method: Effect of moringa leaves ethanol was evaluated in difference day post treatment, day 7th and day 10th. The level of TNF-a and IL-8 from each treatment group in Staphylococcus aureus rhinosinusitis induced rat was evaluated using ELISA compared to control group. Data were analyzed using SPSS 25.0 for Windows. Result: The results of the analysis of different tests of T-Test independence can be concluded that there is decrease in the average level of TNF-α on the 7th day with the 10th day in the P2 and P4 groups there is significant difference (p-value = 0.000), while the P3 group decreased the level of TNF-α on the 7th day with the 10th day there was no significant difference (p-value = 0.630), while the results of the il-8 independence difference in the average il-8 level decrease on the 7th day with the 10th day in Group P2, P3, P4 there is a significant difference (p-value =0.000). Conclusion: The combination of Amoxicillin 27 mg/day and EEDK 100mg/kg is better at decreasing TNF-α and IL-8 levels during 10 days of administration. EEDK can be used as an adjuvant therapy for acute bacterial rhinosinusitis.