Efficient micropropagation of Dendrobium sonia-28 for rapid PLBs proliferation

Abstract

Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved in this study. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid culture. In sucrose treatment, the highest increased of PLBs growth rate percentage was in 10g. L-1. PLBs growth rate percentage was the highest (14%) in media devoid of exogenous 6-benzylaminopurine (BAP) or naphthalene acetic acid (NAA). However, the addition of BAP and NAA in combinations of BAP (4.44 or 8.88 μM) and NAA (8.88 μM) resulted in increased PLBs growth rate to 14% compared to when added separately. The optimized micropropagation media and condition of PLBs proliferation are important for the production of large number of plant material required for the development of genetic modification and cryopreservation studies.