Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis

Abstract

The incorporation of 6 m urea into polyacrylamide-agarose gels reduces the width of the bands formed upon electrophoresis by single-stranded RNA molecules in the 105- to 106.5-dalton size range. As a result the resolving power of gels is increased and molecules that differ in size by as little as 5% can be separated. © 1974.