Combination Simplex Lattice Design Modelling with Chemometrics Analysis for Optimization Formula Self-Nano Emulsion Loaded Quercetin

Abstract

Quercetin is formulated as a self-nano emulsion to improve its solubility and bioavailability. This study focuses on optimizing the components of the SSQ-SNE formula using the SLD method and chemometric analysis. SLD is simple, easy to design, and determines the optimal formula of a constant SNE constituent mixture. The concentration of grapeseed oil (%), surfactant tween 80 (%), and co-surfactant PEG 400 (%) were the components that underwent optimization. The selected formulas consist of grape seed oil at 10.0%, tween 80 at 60.0%, and PEG 400 at 30.0%. The selected formula has a droplet size of 14.2 nm, drug load of 48.53 mg/mL, a viscosity of 666.70 mPa.s, emulsification time at aqua dest 3.71 sec, emulsification time at SGF 3.61 sec, emulsification time at SIF 3.30 sec, a viscosity of 370.147 mPa.s, and a drug load of 31.70 mg/mL. Quercetin was successfully formulated into a self-nano emulsion. The model obtained from optimization can be used to predict the character of the optimum formula.