Effects of Complex Organic Extracts on Callus Growth and PLB Regeneration through Embryogenesis in the Doritaenopsis Orchid

Abstract

Potato extract (PE), corn extract (CE) and papaya extract (PAE) at concentrations of 25, 50 and 100 mL L-1 enhanced callus growth on New Phalaenopsis (NP) medium containing sucrose. Among the various concentrations, 100 mL L-1 PE or PAE and 200 mL L-1 CE significantly promoted callus growth compared to the control. Regeneration of protocorm-like bodies (PLBs) from calli was optimum when NP medium (sometimes referred to as BM) was supplemented with 100 mL L-1 CE, followed by PE and PAE at the same concentration. Most plantlets also regenerated from PLBs with one 100 mL L-1 CE.