Phytochemical Analysis and Antibacterial Efficacy of Mentha piperita (L) Ethanolic Leaf Extract against Clinical Isolates of Uropathogens

Abstract

Aim: The present study was designed to evaluate the antibacterial activity of Mentha piperita (L) leaf ext. against clin. isolates of urinary tract infections. Introduction:M. piperita L. (Peppermint) is a strongly scented herb belonging to family Lamiaceae. The plant is stimulant, arom. and used for headache, vomiting and allaying nausea. In India the leaves are used to relieve sore throat. The most common form of bacterial infections is urinary tract infections (UTIs). They affect people of all age groups throughout their lifespan. Methodol.: The M. piperita ethanolic ext. (MPEE) was prepd. by cold maceration. The presence of phytoconstituents was detd. using std. protocols. Clin. isolates of UTI pathogens such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were isolated from urine samples and identified by biochem. tests. The antibacterial property was detd. by agar well diffusion method. Results and Discussion: The preliminary phytochem. screening revealed the presence of amino acids, carbohydrates, alkaloids, proteins, glycosides, steroids, tannins and flavonoids. MPEE exhibited pronounced antibacterial activity against tested microorganisms. The ext. exhibited antibacterial activity at 1000 μg concn. against S. aureus (21.50 ± 1.22 mm), E. coli (19.33 ± 0.81 mm), P. aeruginosa (15.33 ± 1.69 mm) from high to low resp. The min. inhibitory concn. was ranged from 62.5 to 125 mg/mL. Conclusion: The results of antibacterial studies confirm that MPEE was found to contain bioactive constituents that exhibited notable antibacterial activity. However, further isolation and characterization of phytoconstituents will be needed to evaluate the antimicrobial activities against a wider range of microbial pathogens. [on SciFinder(R)]