IN VIVO ASSESSMENT OF SKIN CHEMICAL BURNS IN EXPOSURE TO VESICANTS AND THE EFFICACY OF AN ANTIDOTE FORMULA IN DIFFERENT PHARMACEUTICAL FORMS. AN EXPERIMENTAL APPROACH

Abstract

The aim of this project was the histopathological and immunofluorescence evaluation of the cutaneous toxicity of the vesicant chemical compound 2-chloroethyl-ethyl sulphide (CEES), a yperite simulator, and the effectiveness of the applied antidote. A complex antidote formula was developed, the treatment offered 100% protection in case of exposure to 1DL50 chemical vesicant. The histopathological evaluation showed that the association of the newly developed antidote with antioxidant and anti-inflammatory actions with the antidotic formulation, in the form of gel, with regenerating, moisturizing and epithelializing actions, is beneficial for 0.25-1 LD50 2-chloroethyl-ethyl sulphide concentrations. Immunofluorescence evaluation of the expression of anti-c-ROS-1 and anti-PARP-1 antibodies, respectively, highlighted the antidysplastic reepithelialising and nuclear stabilizing protective effect of the complex antidote on the skin lesions induced by the studied vesicant compound. The combination of the two complex curative antidotes (A1 formulated as a solution and A2 formulated as a hydrogel) developed within the project has superior therapeutic efficacy. It can guide the therapeutic conduct in case of exposure to vesicant chemicals.