Invaders: Recognition of Double-Stranded DNA by Using Duplexes Modified with Interstrand Zippers of 2′-O-(Pyren-1-yl)methyl-ribonucleotides

Abstract

The invasion has begun: Invaders are shown to recognize DNA hairpins in cell-free assays and chromosomal DNA during non-denaturing fluorescence in situ hybridization (nd-FISH) experiments. As Invaders are devoid of inherent sequence limitations, many previously inaccessible DNA targets could become accessible to exogenous control with important ramifications for karyotyping, in vivo imaging, and gene regulation. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.