Composição química e modulação da resistência bacteriana a drogas do óleo essencial das folhas de Rollinia leptopetala R. E. Fries

Abstract

“Chemical composition and modulation of bacterial drug resistance of the essential oil from the leaves of Rollinia leptopetala R. E. Fries”. The essential oil from the leaves of Rollinia leptopetala was obtained by hydrodistillation in Clevenger’s apparatus and its chemical composition was analyzed by GC-MS. With this technique could be identifi ed 22 constituents in a complex mixture of monoterpenes (54.5%) and sesquiterpenes (45.5%). The main component found in the leaves was the bicyclogermacrene (22.47%). The essential oil was assayed against a strain of Staphylococcus aureus possessing effl ux mechanism of resistance to norfl oxacin. Although the essential oil did not display relevant antibacterial activity in vitro, it modulated the activity of the norfl oxacin, i.e. in combination with the antibiotic it was observed a fourfold reduction in the minimum inhibitory concentration for norfl oxacin, indicating inhibition of effl ux pump.