A new tool for studying waterfowl immune and metabolic responses: Molecular level analysis using kinome profiling

Abstract

Here, we describe the design of an Anas-specific kinome peptide array that can beused to study the immunometabolic responses of mallard and American black duckto pathogens, contaminants, and environmental stress. The peptide arrays contain2,642 unique phosphorylate-able peptide sequences representing 1,900 proteins.These proteins cover a wide array of metabolic and immunological processes, and758 Gene Ontology Biological processes are statistically significantly represented onthe duck peptide array of those 164 contain the term "metabolic" and 25 "immune."In addition, we conducted a comparison of mallard to American black duck at a genetic and proteomic level. Our results show a significant genomic and proteomicoverlap between these two duck species, so that we have designed a cross-reactivepeptide array capable of studying both species. This is the first reported development of a wildlife species-specific kinome peptide array.