First Report of Phomopsis sp. PV-S G38 Caused Leaf Ring Spot Disease on Black Pepper (Pipper nigrum L.) in West Java, Indonesia

Abstract

Phomopsis sp. PV-S G38 have been frequently reported as plant pathogens, non-pathogenic endophytes or saprophytes, commonly infecting several agriculturally important crops. Infection of Phomopsis sp. PV-S G38 on black pepper in Indonesia has never been reported. The purpose of this study was to identify the cause of leaf ring spot disease on black pepper leaves. We used the most efficient method by combining morphological and molecular identification of the pathogen and analyzed the isolates that displayed the symptoms in the field. The primers of ITS 1 and ITS 4 were used. The GelDoc visualization ranged from 500–600 bp. This research discovered that Phomopsis sp. PV-S G38 was a pathogen in black pepper. The symptom of this disease was shown by the appearance of single heliocentric spot on the leaf. This evidence was supported by the sequence report. It revealed that the isolate was 100% identical to Phomopsis sp. PV-S G38.