Recycling of transferrin receptors in A431 cells is inhibited during mitosis.

Abstract

There is a marked reduction in the number of surface transferrin receptors as A431 cells enter mitosis which persists until telophase when receptors reappear to a level that exceeds the original interphase value. This is most simply explained by assuming that recycling of receptors back to the cell surface is inhibited as cells enter mitosis but that internalisation continues for a short while, causing surface receptor depletion. In telophase recycling would resume before internalisation giving a temporary excess of surface transferrin receptors.